Journal: Developmental cell

Article Title: Comprehensive endogenous tagging of basement membrane components reveals dynamic movement within the matrix scaffolding

doi: 10.1016/j.devcel.2020.05.022

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 2017 NK2318 ten-1::LL::mNG CRISPR knock-in; qy56 This study NK2502 ddr-1::LL::mNG CRISPR knock-in; qy43 This study NK2456 ddr-2::LL::mNG CRISPR knock-in; qy44 This study NK2457 ptp-3::LL::mNG CRISPR knock-in; qy47 This study NK2477 sdn-1::LL::mNG CRISPR knock-in; qy29 This study NK2413 dgn-2::LL::mNG CRISPR knock-in; qy 82 This study NK2587 gon-1::LL::mNG CRISPR knock-in; qy45 This study NK2590 mig-17::mNG::3xFlag; shc19 Ji et al. 2019 FDU1506 Oligonucleotides Table S2 Recombinant DNA pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA) Addgene 47550 mNG-C1^SEC^linker This study mNG-SEC-LL mNG-C1^SEC^bothlink This study mNG-SEC-bothlink mEos2-C1^SEC This study mEos2-SEC L4440 RNAi empty vector control Addgene 1654 mig-6 RNAi This study / Ahringer mig-6 RNAi Software and Algorithms Fiji Schindelin et al., 2012 Image J 2.0.0-rc-69/1.52p, http://fiji.sc/ Photoshop Adobe Version CC 2018 Imaris Bitplane Version 9 Illustrator Adobe Version CC 2018 R GNU Project Open Source μManager Edelstein et al., 2010 Version 1.4, https://micromanager.org/ iLas Biovision V.1.2.3 MetaMorph Molecular Devices 7.10.3.279 Excel Microsoft Version 15.39 GraphPad Prism GraphPad Version 7 Open in a separate window KEY RESOURCES TABLE In vivo visualization of 29 basement membrane (BM) proteins fused with mNeonGreen Embryonic BMs share a common template but diversify greatly during development Stable BM proteins form a scaffold that supports movement of dynamic BM components Papilin promotes local BM collagen removal by limiting GON-1 protease localization

Techniques: Virus, Recombinant, Mutagenesis, CRISPR, Knock-In, Clinical Proteomics, Membrane, Plasmid Preparation, Control, Software

Journal: Developmental cell

Article Title: Comprehensive endogenous tagging of basement membrane components reveals dynamic movement within the matrix scaffolding

doi: 10.1016/j.devcel.2020.05.022

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 2017 NK2318 ten-1::LL::mNG CRISPR knock-in; qy56 This study NK2502 ddr-1::LL::mNG CRISPR knock-in; qy43 This study NK2456 ddr-2::LL::mNG CRISPR knock-in; qy44 This study NK2457 ptp-3::LL::mNG CRISPR knock-in; qy47 This study NK2477 sdn-1::LL::mNG CRISPR knock-in; qy29 This study NK2413 dgn-2::LL::mNG CRISPR knock-in; qy 82 This study NK2587 gon-1::LL::mNG CRISPR knock-in; qy45 This study NK2590 mig-17::mNG::3xFlag; shc19 Ji et al. 2019 FDU1506 Oligonucleotides Table S2 Recombinant DNA pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA) Addgene 47550 mNG-C1^SEC^linker This study mNG-SEC-LL mNG-C1^SEC^bothlink This study mNG-SEC-bothlink mEos2-C1^SEC This study mEos2-SEC L4440 RNAi empty vector control Addgene 1654 mig-6 RNAi This study / Ahringer mig-6 RNAi Software and Algorithms Fiji Schindelin et al., 2012 Image J 2.0.0-rc-69/1.52p, http://fiji.sc/ Photoshop Adobe Version CC 2018 Imaris Bitplane Version 9 Illustrator Adobe Version CC 2018 R GNU Project Open Source μManager Edelstein et al., 2010 Version 1.4, https://micromanager.org/ iLas Biovision V.1.2.3 MetaMorph Molecular Devices 7.10.3.279 Excel Microsoft Version 15.39 GraphPad Prism GraphPad Version 7 Open in a separate window KEY RESOURCES TABLE In vivo visualization of 29 basement membrane (BM) proteins fused with mNeonGreen Embryonic BMs share a common template but diversify greatly during development Stable BM proteins form a scaffold that supports movement of dynamic BM components Papilin promotes local BM collagen removal by limiting GON-1 protease localization

Techniques: Virus, Recombinant, Mutagenesis, CRISPR, Knock-In, Clinical Proteomics, Membrane, Plasmid Preparation, Control, Software